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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Laryngeal transplantation in minipigs: early immunological outcomes
doi: 10.1111/j.1365-2249.2011.04531.x
Figure Lengend Snippet: Antibodies, isotypes and combinations used in multiple colour immunofluorescence studies of the laryngeal mucosa. Five combinations, each of three antibodies, were used
Article Snippet: Percentage areas of positive pixel staining were calculated for each fluorochrome and fluorochrome combination. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody † to Clone Isotype Secondary antibody ‡ False colour Combination Myeloid cells CD172 742215 IgG2B IgG2b-FITC a Green 1 CD163 2A/10/11 IgG1 IgG1 - TRITC a Red 1 MHC-II MSA3 EPICS IgG2A IgG2a-AF633 b Blue 1 and 2 CD14 MIL2 IgG2B IgG2b-FITC a Green 2 CD16 c G7 IgG1 IgG1-TRITC a Red 2 Lymphoid cells CD45RC MIL15 IgM IgM-FITC a Green 3 and 4 CD25 K231.3B2 IgG1 IgG1-TRITC a Red 3 and 4 CD4 MIL17 H-M IgG2B IgG2b-AF633 b Blue 3 CD8 MIL12 IgG2A IgG2a-AF633 b Blue 4 and 5
Techniques: Immunofluorescence